讲座：Colouring Labelled Lines: Multispectral Mapping and Activity-Dependent Silencing of Primary Afferents as a Tools to Reveal Distinct Pain and Itch Sensory Lines and to Follow up their Reorganization in Chronic Pain
舒友生老师课题组邀请了耶路撒冷希伯来大学的Dr. Alexander Binshtok做学术报告，欢迎感兴趣的老师和同学参加。讲座信息如下：
讲座题目：Colouring Labelled Lines: Multispectral Mapping and Activity-Dependent Silencing of Primary Afferents as a Tools to Reveal Distinct Pain and Itch Sensory Lines and to Follow up their Reorganization in Chronic Pain
主讲人：Dr. Alexander Binshtok
时 间：4月14日下午16:00
地 点：脑成像中心 308会议室
Alexander Binshtok:
Dr. Alexander Binshtok received his Ph.D. degree in The Hebrew University of Jerusalem in 2006. He worked as postdoctoral fellowship in Neural Plasticity Research Group, Mass General Hospital and Harvard Medical School from 2006 to 2007. After that, he worked as the instructor in Harvard Medical School from 2007 to 2010. From 2011 to the present, he is the PI in Department of Medical Neurobiology, Institute for Medical Research Israel Canada Faculty of Medicine and The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem.
Abstract:
We have recently developed a "Neuronal Positioning System" (NPS) which allow mapping the location of the axonal arbors of many individual neurons simultaneously, at the resolution of individual axons. This method is based on the spectral properties of retrogradely transported dye-labeled vesicles. We inject overlapping regions of a target organ with three or more different colored retrograde tracers. By analyzing the combinations and intensities of the colors in the individual vesicles transported to the cell soma we calculate the projection sites of the neuron’s axon. This neuronal positioning system (NPS) enabled mapping of many axons in a simple automated way. NPS allowed us to define the axonal arborization area of individual neurons. Thus, by “seeing” many axons in the same preparation, it becomes possible to understand how specific neurons in one region are wired to other neuronal types and other regions. It allows better comprehension of the organization and function of neuronal circuits. We are now using this approach to study axonal organization of peripheral afferents in normal condition and neuronal sprouting following nerve injury that underlies development of neuropathic pain. Moreover, to study the physiological role of peripheral afferent axons in coding of sensorial information we have developed a selective silencing approach. By applying charged derivative of lidocaine - QX-314 together with the algogens (pain-inducing agents) and histaminergic and non-histaminergic pruritogens (itch-inducing agents) we were able to selectively block pain and itch respectively. Moreover, we have discovered multiple functionally distinct itch sensing neuronal lines.
